Stretch Chambers

 The exceptional physical and chemical properties of the silicone elastomer PDMS (polydimethylsiloxane) create a specially flexible thin-membrane chamber.

Stretch chambers are flexible culture dishes developed for inducing mechanical stimulation on cultured cells.

  • High reproducibility: Spring-like PDMS chambers bounce back from stretching and compressing. With their original properties intact. The chambers show good reproducibility in applications which need continuous mechanical stretching over prolonged periods of time.
  • Superior transparency: An optically transparent, ultra-thin (100-200 μm) membrane at the well bottom.  It not only makes stretch chambers compatible with optical microscopy techniques, but with fluorescence detection and microscopy as well.

Uniformity of direction and force are issues of crucial concern in cell stretching.
Stretching systems and chambers with insufficient properties can cause extraneous stretching on the wrong axis and generate a secondary load in that direction.
Also, these systems may not apply equal stress to all the cells in the chamber. This makes it difficult to accurately gauge the effect of the stretch stimulus across the unevenly treated sample.
The STREX Cell Stretching System is designed to achieve stretching in a single, parallel direction, with only a weak secondary load.
Research has demonstrated that the STREX system enables highly reproducible cyclic stretching over prolonged periods at ratios of 1-20%. (Ref.)
Because the methyl groups align themselves to the surface, the stretch chamber is highly water-repellent, meaning that cells will not adhere without pre-treatment of the chamber.
Thus, the PDMS chamber surface needs to be coated with extracellular matrix to successfully adhere the culture cells. Fibronectin, collagen, gelatin and laminin, among others, can be used for this purpose.
STREX offers standard stretch chambers in two sizes, the 4 cm² and 10cm² models. Specialized versions are also available, including multi-well, gel-stretching, and transwell experiment chambers.

Ref. Naruse. K., Yamada T., Sai X.R., Hamaguchi M., Sokabe M. (1998), Pp125FAK is required for stretch dependent morphological response of endothelial cells. Oncogene,17:455-463.

Stretch Chambers

4cc Chamber

STB-CH-04STB-CH-04

Corresponding models:
STB-140-04
STB-150
STB-10-04

Culture area:
2.0 x 2.0 x 1.0 cm

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Real Time Imaging

STB-CH-0.02

Corresponding models:
STB-150W

up to 40x magnification

(In order to see at this magnification the motor must be turned off briefly.)

Culture area:
1.0 x 1.0 x 0.02 cm

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Biaxial Stretch Chamber

STB-CH-04-XY

Corresponding models: STB-190-XY

Culture area:
2.0 x 2.0 x 1.0 cm

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10cc Chamber

STB-CH-10

Corresponding models:
STB-140-10
STB-10-10

Culture area:
3.2 x 3.2 x 1.0 cm

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Three Dimensional Culture

STB-CH4-G

Corresponding models:
STB-140-04
STB-10-04

Culture area:
1.0 x 1.0 x 0.02 cm

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Tissue Samples

STB-CH-0.06

Corresponding models:
STB-140-04
STB-140-10

For Pulmonary Tissue

Culture area:
1.0 x 0.6 x 1.0 cm

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STB-CH-4W

Corresponding models:
STB-140-10
STB-10-10

Culture area:
1.5 x 1.5 x 1.0 cm

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STB-CH4-GP

Corresponding models: STB-140-04 STB-10-04

4 microchambers 20 x 20mm

Culture volume: 4cc

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STB-CH-1.5

Corresponding models:
STB-140-04
STB-140-10

For Achilles Tendon

Culture area:
1.5 x 1.0 x 1.5 cm

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Custom chambers available, contact us for details.

Coating Protocol

Stretch Chamber Accessories