Cell Pressure Systems

Simulating Physiological Conditions and Enhancing Biological Research

The Strex Cell Pressure System utilizes a chamber pressurized by our regulator unit to apply pressure to live cells and tissue allowing researchers to simulate in-vivo biochemical activity in cell culture.

Cell Pressure

Cells in their natural environments experience various dynamic forces like shear stress, mechanical strain, and hydrostatic pressure. However, the role of pressure in cellular responses in laboratory settings is often overlooked.

Addressing Research Gap
Remarkably, there are few commercial solutions available to study the effects of pressure on cells. Strex’s Pressure System aims to fill this gap by creating an environment that simplifies the living conditions of cultured cells.

How It Works
Our Pressure System applies pneumatic pressure within a CO₂ chamber to mimic physiological stimuli experienced by cells found in organs such as the bladder, kidneys, and intestines.

Pressure system for cell culture

Advancing Research
This innovative technology provides a simplified yet highly relevant model for studying complex biological functions affected by pressure. Explore recent publications utilizing our technology to advance your research on cellular dynamics under pressure.

Includes:
  • Pressure Chamber
  • Control Box
  • Pressure Regulator
  • Vacuum Tubes
  • Programmed Pressure Patterns
  • Optional CO2 Sensor

*pre-mixed gas supply not included.

Cell culture plates are placed inside of the Strex Pressure Chamber. This chamber is placed inside an incubator and connected to the control box on the outside of the incubator via vacuum tubing that is inserted through the utility port on the incubator. The control box is also connected to a pre-mixed gas supply (not included) via vacuum tubing. Alternatively, the Strex Microscope Mountable Pressure Chamber sits under a microscope inside a glass-bottom culture dish. A microincubator is recommended for temperature control.

The control unit has a fully automated program that can be run in either continuous or cyclic mode with gas exchange occurring at given intervals to ensure cell viability. This 30 second exchange typically occurs every 60-90 minutes by releasing pressure and re-pressurizing the chamber, so you will notice a decrease in pressure during this process. Our system conveniently automates these steps for you and includes many pressure patterns to mimic various physiological conditions.

Contact us for an online demo to learn about the various pressure programs.

Cell Types of Interest:

  • Epithelial Cells (bladder, kidney, etc.)
  • Endothelial Cels
  • Smooth Muscle Cells
  • Retinal Tissue
Research Applications:

  • Mechanotransduction
  • Glaucoma

Research papers referencing the use of our pressure system can be found here.

2023

Beneficial and Detrimental Pressure-Related Effects on Inner Neurons in the Adult Porcine In Vitro Retina.
Svare, F., & Ghosh, F.
(2023).  Translational Vision Science & Technology, 12(2), 19-19.

2022

Biphasic effect of mechanical stress on lymphocyte activation.
Yassouf, M. Y., Zhang, X., Huang, Z., Zhai, D., Sekiya, R., Kawabata, T., & Li, T. S.
(2022).  Journal of Cellular Physiology, 237(2), 1521-1531.

2019

Mechanical stress accompanied with nuclear rotation is involved in the dormant state of mouse oocytes.
Nagamatsu, G., Shimamoto, S., Hamazaki, N., Nishimura, Y., & Hayashi, K.
(2019).  Science Advances, 5(6), eaav9960.

Cell Pressure System, Single Channel

SP-1000

Specifications

Includes control box, square pressure chamber and pressure regulator.

13.3 – 40.0 kPa (100 – 300 mmHg), 1channel, PC Vessel.

Settings: Continuous and cyclic mode.

Continuous Pressure:1 kPa (7.6 mmHg) incremental settings. The pressure is applied until gas exchange.

Cyclic Pressure: Pressure oscillates between two pressure settings. The period of oscillation is set by the user between 1-180 minutes.

Internal Dimensions: L 18.5 x W 16.5 x H 11.6 cm

Note: Model SP-1000 is the replacement model to AGP-1000

Accessories

Microscope Stage-top Vessel
SP-1001

Price: USD 3,740

Note: Model SP-1001 is the replacement model to AGP-3001-M

Custom systems also available by request including applying pressure and electric stimulation. Contact us today to discuss the scope of your research and see how we can assist you.

Microscope-Mountable Pressure Stimulation System

SP-1010

Microscope mountable model for observation of samples in a 35mm culture dish.

Specifications

Includes control box, pressure regulator, microscope-mountable chamber capable of holding a single glass-bottom culture dish.
Can be custom designed to hold up to 6 dishes. Contact us about compatibility.

Microscope, 1ch

Settings: Continuous and cyclic mode.

Stimulation Pressure: 0 – 200 mmHg with 7.6 mmHg incremental settings.
Pressure is applied until gas exchange.

Cyclic Pressure:: Pressure oscillates between two pressure settings, each between
0 – 200 mmHg. The period of oscillation is set by the user between 1-180 minutes.

Note: Model SP-1000 is the replacement model to AGP-1000

Custom systems are also available by request, including applying pressure and electric stimulation.
Contact us today to discuss your research and see how we can assist you.

Discontinued Products

SP-1200 Dual chamber low pressure model for higher throughput.

Previously named AGP-2000

1 Control box, 2 square pressure chambers to accommodate up to 12 multiwell plates or 8 Petri dishes, and pressure regulator.

System Specifications:
100 – 300 mmHg, 2ch, PC Vessel.
Settings: Continuous and cyclic mode.
Stimulation Pressure: 0 – 300 mmHg with 7.6 mmHg incremental settings.
Internal Dimensions (each chamber): L 18.5 x W 16.5 x H 11.6 cm

SP-2000 Single channel high pressure model with a stainless steel chamber.

Previously named AGP-1000-HP

Control box, cylindrical pressure chamber to accommodate up to 3 multiwell plates, and pressure regulator.

System Specifications:

Models:
SP-2000: 100 – 450 mmHg, 1ch, SS Vessel.
SP-2200: 100 – 450 mmHg, 2ch, SS Vessel.

Settings: Continuous and cyclic mode.

Continuous Pressure: 0 – 450 mmHg with 7.6 mmHg incremental settings.
The pressure is applied until gas exchange.

Cyclic Pressure: Pressure oscillates between two pressure settings, each between
0 – 450 mmHg. The period of oscillation is set by the user between 1-180 minutes.

Internal Dimensions: 15 cm Internal Diameter X 11.6 cm Height

Let's Get Started